STED

Enabling STED (Stimulated emission depletion) allows achieving super-resolution on confocal systems that support this feature.

Usage

To activate STED is sufficient to enable the switch, below there is an overview of the settings for each mode:

Device / Mode	Confocal	STED
Depletion laser	OFF	ON
Excitation laser (Red)	Continuous wave (CW)	Pulsed Mode
Lasers trigger	OFF	ON (1MHz)
Excitation polarization waveplate	0% (circular)	100% (linear)

Activating the STED mode is not instantaneous as the polarization waveplate can take few second to change position.

Once the STED mode is activated, the depletion laser power can be adjusted.